Two assay procedures are described in the user guide. The test tube procedure requires a larger volume (0.1 mL) of protein sample; however, it uses a sample to working reagent ratio of 1:20 …
BCA Assay
The macromolecular structure of protein, the number of peptide bonds and the presence of four particular amino acids (cysteine, cystine, tryptophan and tyrosine) are reported to be …
Protocol for Bicinchoninic Acid (BCA) Protein Assay
Learn about the principle of the Bradford protein assay, why it's important, and how to perform it using a step-by-step protocol.
The Thermo ScientificTM PierceTM BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein.
Protein Quantification: BCA Assay – QB3 Berkeley
As the protein concentration increases, more of the BCA-Cu1+ complex will form and the absorbance will increase. This relationship is linear and therefore follows the Beer-Lambert …
The following is a typical standard curve for the BCA Protein Assay when bovine serum albumin is used for the standard. The table gives the actual values for each point on the standard curve …
PREPARATION BEFORE USE NOTE: The BCA and Copper Solutions may precipitate in cold weather or after long term storage, simply warm and stir to re-dissolve.
In a 96-well plate pipette 1ul of BSA standard or sample into a microplate well. After 30 minutes cool the plate to room temperature and read immediately at 562nm absorbance. BSA …
The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure,1 in that both rely on the formation of a Cu2+-protein complex under alkaline conditions, followed by …
The Micro BCA Protein Assay Kit uses concentrated reagents and a protocol that utilizes an extended incubation time at an elevated temperature (60°C, Test Tube Procedure only). The …